Commands:
View > Chromatogram Group
Edit > Cut
View > Chromatogram Group > Alignment

A chromatogram group collects several related chromatographic runs. It is used to align their time axis to each other, to normalise them in the same way or to quantify ions and proteins in all chromatograms simultaneously.

A Chromatogram Group view is generated by the command View > Chromatogram Group. A chromatogram group is used to align the time axis of related chromatograms.

Activities:
  • Adding a chromatogram to the group: dropping the link icon of a spectrum view related to a chromatogram or of a chromatogram map view onto the chromatogram group view
  • Removing a chromatograms from a group: selecting the chromatograms and using the command Edit > Cut
  • Storing text information about the group: dragging the lower border of the table upwards opens a text field
  • Changing the name of the chromatogram group: editing the name field at the lower border of the table
  • Time-aligning un-aligned chromatograms: by using the align button the non-aligned chromatograms are aligned
  • Re-aligning all chromatograms: by pressing the Alt key and the align button all chromatograms are re-aligned

The alignment procedure calculates for every chromatogram a time-axis transformation to transform its eigen-time to a norm-time. For the chromatogram that is displayed in bold red its eigen-time is identical to the norm-time. Even if this chromatogram is deleted the functionality of the alignment remains intact. Via the alignment the retention time of every chromatogram in the group can be transformed into the corresponding retention time of any other chromatogram.

The alignment can be inspected in two ways:
  • The alignment function between reference chromatogram time and the aligned chromatogram time can be visualised by dragging the link icon onto a Data Graph view. This function is not directly editable.
  • The points used for alignment can be inspected and edited by using the command View > Chromatogram Group > Alignment while the chromatogram group is the activated view. This displays an chromatogram alignment view.